Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression. In the bargraphs, the data are shown as the mean ± SD (* P < 0.05).

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Expressing, Gene Expression, Immunohistochemistry, Staining, Western Blot

Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: High expression of UQCRFS1 correlates with cell cycle and apoptosis. ( A , B ) The cell cycle genesets expression difference between UQCRFS1 high and low expression. ( C , D ) Correlation between cell cycle signature and UQCRFS1. ( E , F ) The apoptosis genesets expression difference between UQCRFS1 high and low expression. ( G , H ) Correlation between apoptosis signature and UQCRFS1.

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Expressing

Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: High expression of UQCRFS1 correlates with oxidative phosphorylation and DNA damage. ( A , B ) The DNA damage genesets expression difference between UQCRFS1 high and low expression. ( C , D ) Correlation between DNA damage signature and UQCRFS1. ( E , F ) The oxidative phosphorylation genesets expression difference between UQCRFS1 high and low expression. ( G , H ) Correlation between oxidative phosphorylation signature and UQCRFS1.

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Expressing, Phospho-proteomics

Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: High expression of UQCRFS1 promotes proliferation of OC cells. ( A ) UQCRFS1 expression was determined in four OC cell lines A2780, SKOV3, OVCAR8 and OVCAR3 by western blot. ( B ) Western blot was used to detect the expression pattern of UQCRFS1 in A2780 and OVCAR-8 cells after transfection with siRNA for 24 h (n = 3). ( C ) CCK8 assays were performed to evaluate the proliferation of A2780 and OVCAR8 cells after UQCRFS1 knockdown at 0 h, 24 h, 48 h, 72 h (n = 4). ( D – G )UQCRFS1 knockdown caused G0/G1 phase accumulation, as measured flow cytometry. (t-test for G0/G1 phase and * P < 0.05, ** P < 0.01). ( H – K ) Western blotting was used to detect the expression of CDK2, CDK4 and CyclinD1 in A2780 and OVCAR8 cells after knocking down UQCRFS1. GAPDH was used as internal control (n = 3). In the bargraphs, the data are shown as the mean ± SD (* P < 0.05, ** P < 0.01).

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Expressing, Western Blot, Transfection, Knockdown, Flow Cytometry, Control

Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: Knockdown of UQCRFS1 induces apoptosis of OC cells. ( A – D ) Cells were collected and stained with FITC-conjugated annexin V and PI and subjected to flow cytometry. Knockdown of UQCRFS1 increased the proportion of A2780 and OVCAR8 apoptotic cells (t-test, ** P < 0.01 *** P < 0.001). ( E – H ) The expressions of pro-caspase3, pro-caspase-9, Cyto-c and Bcl-2 were determined after knocking down UQCRFS1 by western blot analysis. GAPDH was used as internal control (n = 3). In the bargraphs, the data are shown as the mean ± SD (* P < 0.05, ** P < 0.01 *** P < 0.001).

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Knockdown, Staining, Flow Cytometry, Western Blot, Control

Journal: Scientific Reports

Article Title: UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer

doi: 10.1038/s41598-023-35572-z

Figure Lengend Snippet: Knockdown of UQCRFS1 triggers changes in the DNA damage genes, ROS production and AKT/mTOR signaling pathway. ( A ) Flow cytometry was used to detect ROS levels in A2780 and OVCAR8 cells after knockdown of UQCRFS1 by loading the DCFH-DA probe, and the peak shifted to the right and ROS production increased (t-test, P < 0.05) ( B ) Relative mRNA expression of ATM, ATR, CHK1 and CHK2 in A2780 and OVCAR8 cells after knockdown of UQCRFS1. ( C ) Correlation between AKT/mTOR signature and UQCRFS1. ( D – G ) Western blot of AKT, p-AKT, mTOR, p-mTOR in siCtrl and siUQCRFS1 about A2780 and OVCAR8 (n = 3). In the bargraphs, the data are shown as the mean ± SD (* P < 0.05, ** P < 0.01 *** P < 0.001).

Article Snippet: Figure 2 High expression of UQCRFS1 correlates with poor disease prognosis in OC. ( A ) Relative level of UQCRFS1 mRNA in ovarian cancer tissues (n = 426) compared with adjacent normal tissues (n = 88) from Gene Expression Profiling Interactive Analysis (GEPIA) online website. ( B ) Summary and representative images of UQCRFS1 immunohistochemistry staining intensities in normal ovarian and OC, based on Human Protein Atlas database (HPA). ( C , D ) UQCRFS1 expression evaluation by western blot analysis in normal ovarian tissue and OC (n = 6). ( E ) Representative IHC images of UQCRFS1 in OC and normal ovarian tissues (Normal = 10, Cancer = 30, Scale bar, 50um). ( F ) Kaplan–Meier curves of overall survival (OS) and progression-free survival (PFS) in ovarian cancer stratified by UQCRFS1 expression.

Techniques: Knockdown, Flow Cytometry, Expressing, Western Blot